Donnerstag, 23. März 2017

BDQ at the Gene Quantification Event

BDQ at the Gene Quantification Event
Biomolecular Detection and Quantification is a proud sponsor of the qPCR dPCR & NGS, Gene Quantification Event, taking place 3-7 April 2017 in Freising, Germany. Leading experts will present on quantitative RT-PCR, dPCR, Next Generation Sequencing technologies, integrative big data analysis, and more. Visit the event website for the full agenda and additional information.
Meet the Editors
Visit the BDQ booth in the exhibition hall to:
Discover the Editorial Board
Unable to make it to the event?
Read the BDQ Special Issue on digital PCR. The articles in this Special Issue illustrate the potential of this powerful technique to advance clinical research and routine diagnosis with its reproducibility and improved sensitivity.
Read the Special Issue on dPCR

Mittwoch, 22. März 2017

Last chance to register @ qPCR dPCR & NGS 2017

8th Gene Quantification Event -- qPCR dPCR & NGS 2017
72 Talks and 78 Posters are accepted for presentation

Symposium Agenda (online version) =>
(PDF version for download) =>
Poster Session (online version) =>
(PDF version for download) =>

Scientific Symposium Sessions:
Liquid Biopsies & Exosomes --
Liquid Biopsies & Molecular Diagnostics -- and
NGS Integrative Data Analysis --
digital PCR -- and
• Next Generation Sequencing --
non-coding RNAs --
MIQE & qPCR Quality Control --
qPCR Data Analysis --
MicroGenomics & Single-Cell-qPCR --

Dienstag, 21. März 2017

Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper

Obstacles and opportunities in the functional analysis of extracellular vesicle RNA – an ISEV position paper

Bogdan MateescuEmma J. K. KowalBas W. M. van BalkomSabine BartelSuvendra N. BhattacharyyaEdit I. BuzásAmy H. BuckPaola de CandiaFranklin W. N. ChowSaumya DasTom A. P. DriedonksLola Fernández-MessinaFranziska HaderkAndrew F. HillJennifer C. Jones,Kendall R. Van Keuren-JensenCharles P. LaiCecilia LässerItalia di LiegroTaral R. LunavatMagdalena J. LorenowiczSybren L. N. MaasImre MägerMaria MittelbrunnStefan MommaKamalika MukherjeeMuhammed NawazD. Michiel PegtelMichael W. PfafflRaymond M. Schiffelers,Hidetoshi TaharaClotilde ThéryJuan Pablo TosarMarca H. M. WaubenKenneth W. Witwer and Esther N. M. Nolte-‘t Hoen

The release of RNA-containing extracellular vesicles (EV) into the extracellular milieu has been demonstrated in a multitude of different in vitro cell systems and in a variety of body fluids. RNA-containing EV are in the limelight for their capacity to communicate genetically encoded messages to other cells, their suitability as candidate biomarkers for diseases, and their use as therapeutic agents. Although EV-RNA has attracted enormous interest from basic researchers, clinicians, and industry, we currently have limited knowledge on which mechanisms drive and regulate RNA incorporation into EV and on how RNA-encoded messages affect signalling processes in EV-targeted cells. Moreover, EV-RNA research faces various technical challenges, such as standardisation of EV isolation methods, optimisation of methodologies to isolate and characterise minute quantities of RNA found in EV, and development of approaches to demonstrate functional transfer of EV-RNA in vivo. These topics were discussed at the 2015 EV-RNA workshop of the International Society for Extracellular Vesicles. This position paper was written by the participants of the workshop not only to give an overview of the current state of knowledge in the field, but also to clarify that our incomplete knowledge – of the nature of EV(-RNA)s and of how to effectively and reliably study them – currently prohibits the implementation of gold standards in EV-RNA research. In addition, this paper creates awareness of possibilities and limitations of currently used strategies to investigate EV-RNA and calls for caution in interpretation of the obtained data.

Sonntag, 19. März 2017

Reproducibility of biomedical research – The importance of editorial vigilance.

Reproducibility of biomedical research – The importance of editorial vigilance.
Stephen A. Bustin & Jim F. Huggett
Biomolecular Detection and Quantification, March 2017, Vol.11: 1–3

Many journal editors are a failing to implement their own authors’ instructions, resulting in the publication of many articles that do not meet basic standards of transparency, employ unsuitable data analysis methods and report overly optimistic conclusions. This problem is particularly acute where quantitative measurements are made and results in the publication of papers that lack scientific rigor and contributes to the concerns with regard to the reproducibility of biomedical research. This hampers research areas such as biomarker identification, as reproducing all but the most striking changes is challenging and translation to patient care rare.

Mittwoch, 1. März 2017

Our new paper in Nature Methods -- EV-TRACK

Our new paper in Nature Methods
"EV-TRACK: transparent reporting & centralizing knowledge in extracellular vesicle research"


We argue that the field of extracellular vesicle (EV) biology needs more transparent reporting to facilitate interpretation and replication of experiments. To achieve this, we describe EV-TRACK, a crowdsourcing knowledgebase ( that centralizes EV biology and methodology with the goal of stimulating authors, reviewers, editors and funders to put experimental guidelines into practice.