Samstag, 24. September 2016


Topic: Diet, Environment and Extracellular Vesicles
Date: 27-28 January, 2017
Free registrations are open now:
For more information, visit the ISEV webpage:

Chair: Suresh Mathivanan (La Trobe University, Australia)
Local and International Board:  Michael Pfaffl (Technical University of Munich, Germany); Marilyn Anderson (La Trobe University, Australia); Mark Bleackley (La Trobe University, Australia)
ISEV Board: Marca Wauben (Utrecht University, The Netherlands); Andrew Hill (La Trobe University, Australia)

As the workshop precedes the Australasian EV meeting (31 Jan - 2 Feb, 2016), travel to Australia and attend two exciting workshop/conferences on EVs.

Freitag, 23. September 2016

qPCR NEWS Sept. 2017 -- Event & Workshop Calendar

Dear researcher,
dear Gene Quantification page reader,
Our newsletter informs about the latest news in gene expression profiling using qPCR and related methods, which are compiled and summarised on
The focus of this qPCR NEWS issue is:

Freitag, 19. August 2016

Is there a cross-kingdom microRNA transfer?

Is there a cross-kingdom microRNA transfer?
Join our August 2016 newsletter with focus on "Cross-kingdom microRNA transfer"

REVIEW -- New roles for microRNAs in cross-species communication.
Liang H, Zen K, Zhang J, Zhang CY, Chen X.
RNA Biol. 2013 Mar;10(3): 367-370

Communication between cells ensures coordinated behavior. In prokaryotes, this signaling is typically referred to as quorum sensing, whereas in eukaryotic cells, communication occurs through hormones. In recent years, reports have shown that small noncoding RNAs, called microRNAs (miRNAs), can be transmitted from one species to another, inducing signal interference in distant species, even in a cross-kingdom manner. This new mode of cross-species communication might mediate symbiotic and pathogenic relationships between various organisms (e.g., microorganisms and their hosts). Here, we discuss several recent studies concerning miRNA-mediated cross-species gene regulation.

Dienstag, 2. August 2016

qPCR dPCR NGS 2017 -- Event promotion trailer

qPCR dPCR & NGS 2017
8th international Gene Quantification Event
Symposium & Industrial Exhibition & Application Workshops
Main topics:   
Liquid Biopsy, Integrative Big Data Analysis, Biomarker Signature … and beyond
3 - 7 April 2017, in Freising-Weihenstephan,
School of Life Sciences, Technical University of Munich, Weihenstephan, Germany

Register and submit your scientific contribution, talk or poster abstract =>

Donnerstag, 28. Juli 2016

How to apply the MIQE guidelines - a visual, interactive and practical qPCR guide

2nd edition published 26th July 2016
Editors:  Afif M. Abdel Nour & Michael W. Pfaffl
ISBN 9783000488061
Free download via iTunes

more info here

MIQE & qPCR Preface:   The qPCR and dPCR MIQE guidelines – A success story!
(Editorial for 2nd edition published in July 2016)

The MIQE guidelines and the resulting scientific validity will be supported by more and more researchers, biological journals, academic and commercial institutions. Today in July 2016 we count more than 4600 citations for the MIQE guideline applied in qPCR and around160 citations for the digital PCR (dPCR) MIQE guideline (measured by Google Scholar). Hence the qPCR and dPCR MIQE guidelines are a worldwide full success story which will be driven forward by the scientific community, e.g.

The present second edition of the MIQE & qPCR iBook should help to spread this MIQE idea even further in any laboratory worldwide and beyond in the scientists’ workflow and minds. It should clearly show how to apply the guidelines and serve as a handy, visual, interactive and practical guide. For now in the first year after publication of the first edition we could count more than 1200 downloads of the MIQE & qPCR iBook from more than 30 countries. Our goal for the second edition is to update the existing content by new chapters, and to improve this fancy interactive tool, interfacing scientific publications with educating pictures, videos and scientific talks. We implemented multiple new chapters, describing the significance of the reverse transcription reaction, why PCR assay validation is so important for high sensitivity and good reproducibility, and one reviewing chapter about the necessity of performing quality control at all levels in the qPCR workflow. In summary we are proud to present a selection of international highly recognized authors from the academic field as well from industrial research presenting their latest applications. Described qPCR / dPCR methods and applications are linked to the MIQE context and show it on the basis of educational questionnaires or interactive ‘how to do’ instruction sheets. The at-hand MIQE & qPCR iBook should deliver the MIQE guidelines directly to the researcher and help to solve the daily problems in the molecular biology laboratory using quantitative PCR, digital PCR, single-cell qPCR, microRNA applications or any comparable techniques using PCR.

We hope you like our explanatory, interactive and educational iBook concept, showing the advantages of the MIQE guidelines in an easy and understandable way, and to guarantee the successful qPCR or dPCR application at the lab bench.

The editors
Afif M. Abdel Nour & Michael W. Pfaffl