Montag, 8. Mai 2017

Methods to determine limit of detection and limit of quantification in qPCR

Abstract:  Quantitative Real-Time Polymerase Chain Reaction, better known as qPCR, is the most sensitive and specific technique we have for the detection of nucleic acids. Even though it has been around for more than 30 years and is preferred in research applications, it has yet to win broad acceptance in routine practice. This requires a means to unambiguously assess the performance of specific qPCR analyses. Here we present methods to determine the limit of detection (LoD) and the limit of quantification (LoQ) as applicable to qPCR. These are based on standard statistical methods as recommended by regulatory bodies adapted to qPCR and complemented with a novel approach to estimate the precision of LoD.
Keywords:  Limit of detection; Limit of quantification; LoD; LoQ; GenEx software; Real-time PCR; qPCR; Data analysis; Replicates; MIQE; Quality control; Standardization




Sonntag, 9. April 2017

First impressions from a great qPCR dPCR & NGS 2017 Event



First impressions from a great symposium!

8th Gene Quantification Event

qPCR dPCR NGS 2017
150 contributions were presented -- 72 Talks and 78 Posters
Many thanks to all 450 participants from 35 countries
.. and in particular the all speakers, poster presenters and session chairs.
... see you at our next event in 2019 @ TUM Weihenstephan

Donnerstag, 23. März 2017

BDQ at the Gene Quantification Event

Elsevier
BDQ at the Gene Quantification Event
cover
Biomolecular Detection and Quantification is a proud sponsor of the qPCR dPCR & NGS, Gene Quantification Event, taking place 3-7 April 2017 in Freising, Germany. Leading experts will present on quantitative RT-PCR, dPCR, Next Generation Sequencing technologies, integrative big data analysis, and more. Visit the event website for the full agenda and additional information.
Meet the Editors
Visit the BDQ booth in the exhibition hall to:
Discover the Editorial Board
Unable to make it to the event?
Read the BDQ Special Issue on digital PCR. The articles in this Special Issue illustrate the potential of this powerful technique to advance clinical research and routine diagnosis with its reproducibility and improved sensitivity.
Read the Special Issue on dPCR

Mittwoch, 22. März 2017

Last chance to register @ qPCR dPCR & NGS 2017

8th Gene Quantification Event -- qPCR dPCR & NGS 2017
72 Talks and 78 Posters are accepted for presentation
Registration.qPCR-dPCR-NGS-2017.net

Symposium Agenda (online version) => http://Agenda.qPCR-dPCR-NGS-2017.net
(PDF version for download) => http://AgendaPDF.qPCR-dPCR-NGS-2017.net
Poster Session (online version) => http://Posters.qPCR-dPCR-NGS-2017.net
(PDF version for download) => http://PostersPDF.qPCR-dPCR-NGS-2017.net

Scientific Symposium Sessions:
Liquid Biopsies & Exosomes -- https://www.conftool.com/qpcr-dpcr-ngs-2017/index.php?page=browseSessions&form_session=2
Liquid Biopsies & Molecular Diagnostics -- https://www.conftool.com/qpcr-dpcr-ngs-2017/index.php?page=browseSessions&form_session=41 and https://www.conftool.com/qpcr-dpcr-ngs-2017/index.php?page=browseSessions&form_session=3
NGS Integrative Data Analysis -- https://www.conftool.com/qpcr-dpcr-ngs-2017/index.php?page=browseSessions&form_session=1
digital PCR -- https://www.conftool.com/qpcr-dpcr-ngs-2017/index.php?page=browseSessions&form_session=4 and https://www.conftool.com/qpcr-dpcr-ngs-2017/index.php?page=browseSessions&form_session=33
• Next Generation Sequencing -- https://www.conftool.com/qpcr-dpcr-ngs-2017/index.php?page=browseSessions&form_session=36
non-coding RNAs -- https://www.conftool.com/qpcr-dpcr-ngs-2017/index.php?page=browseSessions&form_session=12
MIQE & qPCR Quality Control -- https://www.conftool.com/qpcr-dpcr-ngs-2017/index.php?page=browseSessions&form_session=11
qPCR Data Analysis -- https://www.conftool.com/qpcr-dpcr-ngs-2017/index.php?page=browseSessions&form_session=32
MicroGenomics & Single-Cell-qPCR -- https://www.conftool.com/qpcr-dpcr-ngs-2017/index.php?page=browseSessions&form_session=13